Immobilised enzymes
نویسندگان
چکیده
منابع مشابه
Effect of pore size on the performance of immobilised enzymes.
Porous materials are widely employed as supports in the immobilisation of enzymes. Traditionally macroporous materials with pore diameters >50 nm were believed to be the most suitable support material, ensuring no spatial restrictions upon enzyme molecules entering such large pores. In recent years however, there has been growing emphasis in the use of mesoporous supports with pore diameters ra...
متن کاملEnhancing the Ability Of Keratinase By Using Immobilised Enzymes
Keratinases are a group of proteolytic enzymes that display the capability of degrading insoluble keratin substrates such as feather resulting as poultry waste. Entrapment of crude/purified enzyme within a matrix is a common method for immobilization. An immobilized enzyme provides increased resistance to changes in conditions such as pH or temperature. It also allows enzymes to be held in plac...
متن کاملBIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS Immobilised whole-cell recombinant monoamine oxidase biocatalysis
This work demonstrates the first example of the immobilisation of MAO-N whole cells to produce a biocatalyst that remained suitable for repetitive use after 11 months of storage and stable up to 15 months after immobilisation. The production ofEscherichia coli expressing recombinant MAON was scaled up to bioreactors under regulated, previously optimised conditions (10 % DO, pH 7), and the amoun...
متن کاملImmobilised Nucleotides and Coenzymes for Affinity Ghromatography
General methods for the preparation of coenzyme and nucleo tide analogues substituted with spacer molecules at various positions on the purine, phosphate and ribose moieties and suitable for attachment to insoluble support matrices are given. The relative efficiencies of the resulting differently immobilised nucleotides are compared both in regard to the position of substitution and in. terms o...
متن کاملEffect of intermittent stretch on immobilised muscle.
When muscle is immobilised in a shortened position there is a reduction in fibre length and an increase in the proportion of connective tissue. This results in reduced muscle compliance and a loss of range of joint motion. Experiments have been carried out to determine whether short periods of stretch are effective in preventing these changes. The soleus muscle of the mouse was immobilised in a...
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ژورنال
عنوان ژورنال: Nature
سال: 1979
ISSN: 0028-0836,1476-4687
DOI: 10.1038/280852b0